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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Unidades Centrais. |
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Data corrente: |
03/02/2016 |
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Data da última atualização: |
03/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHWARZ, D. G. G.; PIETRALONGA, P. A. G.; SOUZA, M. C. C.; CARVALHO, I. A.; CRUZEIRO, R. S.; MALAQUIAS, J. V.; BENJAMIM, L. A.; SILVA JÚNIOR, A.; MOREIRA, M. A. S. |
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Afiliação: |
JUACI VITORIA MALAQUIAS, CPAC. |
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Título: |
Cytokine gene expression and molecular detection of Mycobacterium avium subspecies paratuberculosis in organs of experimentally infected mice. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 5, p. 396-402, maio 2015. |
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ISSN: |
0100-736X |
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DOI: |
http://dx.doi.org/10.1590/S0100-736X201500050000 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Resumo - Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos de camundongos C57BL/6 infectados por via intraperitoneal durante 120 dias. Dentre os órgãos avaliados, o baço (85%), cólon (75%) e fígado (60%) tiveram as maiores frequências de positividade. Quando comparadas essas frequências entre os órgãos, verificou-se que o baço teve 1,54 vezes mais probabilidade de ser positivo em relação ao íleo, e 2,0 vezes mais probabilidade em relação às placas de Peyer. Além disso, aos 60 dias pós infecção, o baço e o fígado foram responsáveis pela maior expressão de IFN-γ e o íleo pela TNF-α e IL-4. Os resultados indicam que o baço é o melhor órgão para avaliar uma infecção experimental por MAP, principalmente nos períodos iniciais da infecção. Além disso, demonstrou que o baço, fígado e íleo têm importância direta na resposta inflamatória de modelos experimentais. Abstract - Mycobacterium avium subspecies paratuberculosis (MAP) can infect ruminants and remain subclinical for long periods within herds. The identification of organs that are more susceptible to infection and the evaluation of cytokine expression at the site of infection are important to understand the pathogenesis of MAP. In this study, the probability of detection of MAP-DNA and the expression of cytokines in organs of C57BL/6 mice infected intraperitoneally for 120 days were evaluated. Among the evaluated organs, the spleen (85%), colon (75%) and liver (60%) had the highest frequency of positivity. When compared these frequencies between organs, it has been found that the spleen had 1.54 times as likely to be positive in relation to the ileum, and 2.0 times more likely in relation to the Peyer?s patches. In addition, at 60 days post-infection, the spleen and the liver were responsible for upregulation of IFN-γ , and the ileum by TNF-α and IL-4. The results indicate that the spleen is the best organ for evaluating an experimental infection by MAP, especially in the initial stages of the infection. Moreover, it showed that the spleen, liver and ileum have a direct role in the inflammatory response in experimental models. MenosResumo - Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos de camundongos C57BL/6 infectados por via intraperitoneal durante 120 dias. Dentre os órgãos avaliados, o baço (85%), cólon (75%) e fígado (60%) tiveram as maiores frequências de positividade. Quando comparadas essas frequências entre os órgãos, verificou-se que o baço teve 1,54 vezes mais probabilidade de ser positivo em relação ao íleo, e 2,0 vezes mais probabilidade em relação às placas de Peyer. Além disso, aos 60 dias pós infecção, o baço e o fígado foram responsáveis pela maior expressão de IFN-γ e o íleo pela TNF-α e IL-4. Os resultados indicam que o baço é o melhor órgão para avaliar uma infecção experimental por MAP, principalmente nos períodos iniciais da infecção. Além disso, demonstrou que o baço, fígado e íleo têm importância direta na resposta inflamatória de modelos experimentais. Abstract - Mycobacterium avium subspecies paratuberculosis (MAP) can infect ruminants and remain subclinical for long periods within herds. The identification of organs that are more susceptible to infection and the evaluation of cytokine expression at the sit... Mostrar Tudo |
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Palavras-Chave: |
Doença de Johne; Experimental infection; Infecção experimental; Johne's disease; Johne’s disease; Murinos. |
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Thesagro: |
Paratuberculose. |
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Thesaurus Nal: |
Mice; Mycobacterium avium; Paratuberculosis. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138378/1/Juaci-Cytokine-gene.pdf
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Marc: |
LEADER 03699naa a2200361 a 4500
001 2036032
005 2016-02-03
008 2015 bl uuuu u00u1 u #d
022 $a0100-736X
024 7 $ahttp://dx.doi.org/10.1590/S0100-736X201500050000$2DOI
100 1 $aSCHWARZ, D. G. G.
245 $aCytokine gene expression and molecular detection of Mycobacterium avium subspecies paratuberculosis in organs of experimentally infected mice.
260 $c2015
520 $aResumo - Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos de camundongos C57BL/6 infectados por via intraperitoneal durante 120 dias. Dentre os órgãos avaliados, o baço (85%), cólon (75%) e fígado (60%) tiveram as maiores frequências de positividade. Quando comparadas essas frequências entre os órgãos, verificou-se que o baço teve 1,54 vezes mais probabilidade de ser positivo em relação ao íleo, e 2,0 vezes mais probabilidade em relação às placas de Peyer. Além disso, aos 60 dias pós infecção, o baço e o fígado foram responsáveis pela maior expressão de IFN-γ e o íleo pela TNF-α e IL-4. Os resultados indicam que o baço é o melhor órgão para avaliar uma infecção experimental por MAP, principalmente nos períodos iniciais da infecção. Além disso, demonstrou que o baço, fígado e íleo têm importância direta na resposta inflamatória de modelos experimentais. Abstract - Mycobacterium avium subspecies paratuberculosis (MAP) can infect ruminants and remain subclinical for long periods within herds. The identification of organs that are more susceptible to infection and the evaluation of cytokine expression at the site of infection are important to understand the pathogenesis of MAP. In this study, the probability of detection of MAP-DNA and the expression of cytokines in organs of C57BL/6 mice infected intraperitoneally for 120 days were evaluated. Among the evaluated organs, the spleen (85%), colon (75%) and liver (60%) had the highest frequency of positivity. When compared these frequencies between organs, it has been found that the spleen had 1.54 times as likely to be positive in relation to the ileum, and 2.0 times more likely in relation to the Peyer?s patches. In addition, at 60 days post-infection, the spleen and the liver were responsible for upregulation of IFN-γ , and the ileum by TNF-α and IL-4. The results indicate that the spleen is the best organ for evaluating an experimental infection by MAP, especially in the initial stages of the infection. Moreover, it showed that the spleen, liver and ileum have a direct role in the inflammatory response in experimental models.
650 $aMice
650 $aMycobacterium avium
650 $aParatuberculosis
650 $aParatuberculose
653 $aDoença de Johne
653 $aExperimental infection
653 $aInfecção experimental
653 $aJohne's disease
653 $aJohne’s disease
653 $aMurinos
700 1 $aPIETRALONGA, P. A. G.
700 1 $aSOUZA, M. C. C.
700 1 $aCARVALHO, I. A.
700 1 $aCRUZEIRO, R. S.
700 1 $aMALAQUIAS, J. V.
700 1 $aBENJAMIM, L. A.
700 1 $aSILVA JÚNIOR, A.
700 1 $aMOREIRA, M. A. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 35, n. 5, p. 396-402, maio 2015.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
03/03/2017 |
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Data da última atualização: |
03/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SANTANA, M. H. de A.; OLIVEIRA JUNIOR, G. A.; CESAR, A. S. M.; FREUA, M. C.; GOMES, R. da C.; SILVA, S. da L.; LEME, P. R.; FUKUMASU, H.; CARVALHO, M. E.; VENTURA, R. V.; COUTINHO, L. L.; KADARMIDEEN, H. N.; FERRAZ, J. B. S. |
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Afiliação: |
MIGUEL HENRIQUE DE ALMEIDA SANTANA; GERSON ANTÔNIO OLIVEIRA JUNIOR; ALINE SILVA MELLO CESAR; MATEUS CASTELANI FREUA; RODRIGO DA COSTA GOMES, CNPGC; SAULO DA LUZ E SILVA; PAULO ROBERTO LEME; HEIDGE FUKUMASU; MINOS ESPERÂNDIO CARVALHO; RICARDO VIEIRA VENTURA; LUIZ LEHMANN COUTINHO; HAJA N. KADARMIDEEN; JOSÉ BENTO STERMAN FERRAZ. |
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Título: |
Copy number variations and genome-wide associations reveal putative genes and metabolic pathways involved with the feed conversion ratio in beef cattle. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Applied Gentics, v. 57, n. 4, p. 495-504, 2016 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of genome-wide association results combined with other genomic approaches may uncover genes and metabolic pathways related to complex traits. In this study, the phenotypic and genotypic data of 1475 Nellore (Bos indicus) cattle and 941,033 single nucleotide polymorphisms (SNPs) were used for genome-wide association study (GWAS) and copy number variations (CNVs) analysis in order to identify candidate genes and putative pathways involved with the feed conversion ratio (FCR). The GWAS was based on the Bayes B approach analyzing genomic windows with multiple regression models to estimate the proportion of genetic variance explained by each window. The CNVs were detected with PennCNV software using the log R ratio and B allele frequency data. CNV regions (CNVRs) were identified with CNVRuler and a linear regression was used to associate CNVRs and the FCR. Functional annotation of associated genomic regions was performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) and the metabolic pathways were obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG). We showed five genomic windows distributed over chromosomes 4, 6, 7, 8, and 24 that explain 12 % of the total genetic variance for FCR, and detected 12 CNVRs (chromosomes 1, 5, 7, 10, and 12) significantly associated [false discovery rate (FDR) < 0.05] with the FCR. Significant genomic regions (GWAS and CNV) harbor candidate genes involved in pathways related to energetic, lipid, and protein metabolism. The metabolic pathways found in this study are related to processes directly connected to feed efficiency in beef cattle. It was observed that, even though different genomic regions and genes were found between the two approaches (GWAS and CNV), the metabolic processes covered were related to each other. Therefore, a combination of the approaches complement each other and lead to a better understanding of the FCR. MenosThe use of genome-wide association results combined with other genomic approaches may uncover genes and metabolic pathways related to complex traits. In this study, the phenotypic and genotypic data of 1475 Nellore (Bos indicus) cattle and 941,033 single nucleotide polymorphisms (SNPs) were used for genome-wide association study (GWAS) and copy number variations (CNVs) analysis in order to identify candidate genes and putative pathways involved with the feed conversion ratio (FCR). The GWAS was based on the Bayes B approach analyzing genomic windows with multiple regression models to estimate the proportion of genetic variance explained by each window. The CNVs were detected with PennCNV software using the log R ratio and B allele frequency data. CNV regions (CNVRs) were identified with CNVRuler and a linear regression was used to associate CNVRs and the FCR. Functional annotation of associated genomic regions was performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) and the metabolic pathways were obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG). We showed five genomic windows distributed over chromosomes 4, 6, 7, 8, and 24 that explain 12 % of the total genetic variance for FCR, and detected 12 CNVRs (chromosomes 1, 5, 7, 10, and 12) significantly associated [false discovery rate (FDR) < 0.05] with the FCR. Significant genomic regions (GWAS and CNV) harbor candidate genes involved in pathways related to energetic, lipid,... Mostrar Tudo |
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Palavras-Chave: |
GWAS; Nellore cattle. |
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Thesaurus NAL: |
Cattle; Cucumber necrosis virus; Feed conversion; Genomics; Single nucleotide polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157039/1/Copy-number-variations-and-genome-wide.pdf
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Marc: |
LEADER 03009naa a2200349 a 4500
001 2066092
005 2017-03-03
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTANA, M. H. de A.
245 $aCopy number variations and genome-wide associations reveal putative genes and metabolic pathways involved with the feed conversion ratio in beef cattle.$h[electronic resource]
260 $c2016
520 $aThe use of genome-wide association results combined with other genomic approaches may uncover genes and metabolic pathways related to complex traits. In this study, the phenotypic and genotypic data of 1475 Nellore (Bos indicus) cattle and 941,033 single nucleotide polymorphisms (SNPs) were used for genome-wide association study (GWAS) and copy number variations (CNVs) analysis in order to identify candidate genes and putative pathways involved with the feed conversion ratio (FCR). The GWAS was based on the Bayes B approach analyzing genomic windows with multiple regression models to estimate the proportion of genetic variance explained by each window. The CNVs were detected with PennCNV software using the log R ratio and B allele frequency data. CNV regions (CNVRs) were identified with CNVRuler and a linear regression was used to associate CNVRs and the FCR. Functional annotation of associated genomic regions was performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) and the metabolic pathways were obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG). We showed five genomic windows distributed over chromosomes 4, 6, 7, 8, and 24 that explain 12 % of the total genetic variance for FCR, and detected 12 CNVRs (chromosomes 1, 5, 7, 10, and 12) significantly associated [false discovery rate (FDR) < 0.05] with the FCR. Significant genomic regions (GWAS and CNV) harbor candidate genes involved in pathways related to energetic, lipid, and protein metabolism. The metabolic pathways found in this study are related to processes directly connected to feed efficiency in beef cattle. It was observed that, even though different genomic regions and genes were found between the two approaches (GWAS and CNV), the metabolic processes covered were related to each other. Therefore, a combination of the approaches complement each other and lead to a better understanding of the FCR.
650 $aCattle
650 $aCucumber necrosis virus
650 $aFeed conversion
650 $aGenomics
650 $aSingle nucleotide polymorphism
653 $aGWAS
653 $aNellore cattle
700 1 $aOLIVEIRA JUNIOR, G. A.
700 1 $aCESAR, A. S. M.
700 1 $aFREUA, M. C.
700 1 $aGOMES, R. da C.
700 1 $aSILVA, S. da L.
700 1 $aLEME, P. R.
700 1 $aFUKUMASU, H.
700 1 $aCARVALHO, M. E.
700 1 $aVENTURA, R. V.
700 1 $aCOUTINHO, L. L.
700 1 $aKADARMIDEEN, H. N.
700 1 $aFERRAZ, J. B. S.
773 $tJournal of Applied Gentics$gv. 57, n. 4, p. 495-504, 2016
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Embrapa Gado de Corte (CNPGC) |
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